The higher the level of genetic similarity between the virus causing the first infection and the virus causing the infection pressure afterwards, the more efficiently the mechanism will work. The active substance of PMV®-01 contains a mild isolate of the Chilean PepMV strain. This mild and stable Chilean isolate quickly installs itself in all the parts of the plant, so that the immune system will be activated in the entire crop 4 to 6 weeks after vaccination. After this period the plant is protected against future attacks of aggressive PepMV-isolates. Moreover, the mild isolate causes no symptoms on the fruits and has no effect on production, nor on quality.